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restriction mapping meaning in English

限制制图

Examples

  1. The restriction map of phzl318 carrying the entire add gene cluster from s . avermitilis nrrl8165 was made . its two subclones phz2104 and phz2105 were introduced into s . lividans mutant zx1
    制作了携带完整add基因簇的phz1318的限制内切酶图谱,根据酶谱进行亚克隆得到phz2104和phz2105 。
  2. By comparing restriction maps of plasmids possessed in these clones , 5 clones ( clones 1 , 4 , 5 , 6 , and 8 ) were found to contain the same chitinase gene , while the other three clones ( clones 2 , 3 and 9 ) contain different chitinase genes one another
    经底物反应和限制性内切酶图谱分析,确定其中的clone - 1 , 4 , 5 , 6 , 8含有相同的几丁质酶基因;而clone - 2 , 3 , 5 , 9四株重组菌则含有不同的几丁质酶基因。
  3. The restriction map of the plasmid pbl29 was tentatively constructed according as the molecular weights of fragments of plasmid pbl29 digested with different enzymes . analysing the sequence of the plasmid pbl29 tested , we found that the mol ecular weight of the plasmid pbl29 is 371 lbp , that it s many unique restriction endonucleases , km resistance genes and abundant a / t base pairs of replicating site are on the plasmid pbl29
    同时根据限制性酶切各片段的分子量作出了质粒pbl29的内切酶图谱。并对质粒pbl29进行测序和分析,证明了质粒pbl29大小为3711bp ,具有大量的单酶切位点、编码卡那霉素抗性基因以及富含a t序列的复制起点。
  4. Then , the plasmid was transformed into jm109 . the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence . test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program
    利用rt - pgr技术,设计一对引物,对田间采集的经鉴定含pstvd的阳性样品进行全序列扩增,并将所得产物纯化回收,连接到pmd18t - vector中,并转化至大肠杆菌jm109中,挑选白斑进行双酶切( saci / ecorv )鉴定证明插入片段为359bp大小,进行序列测定,所得克隆基因为pstvd全序列负链,大小为359bp 。

Related Words

  1. wellbore restriction
  2. dividend restrictions
  3. immigration restrictions
  4. contextual restriction
  5. clearance restriction
  6. caloric restriction
  7. restriction area
  8. immune restriction
  9. trade restrictions
  10. restriction loss
  11. restriction map
  12. restriction modification system
  13. restriction mutant
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